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. Author manuscript; available in PMC: 2017 Oct 19.
Published in final edited form as: Bioconjug Chem. 2017 Feb 15;28(4):933–943. doi: 10.1021/acs.bioconjchem.6b00660

Figure 7.

Figure 7

Reaction kinetics for Exo III digestion and DNase I hydrolysis of SH-2X probe DNA-modified AuNPs. (A) Time-dependent fluorescence changes for AuNPs modified with 79 oligonucleotides per particle upon addition of either 80 nM Exo III (0.2 U/μL) or DNase I (0.125 U/μL) in the presence of 200 nM cDNA. (B) Surface coverage measurements obtained for DNA-modified AuNPs with different amounts of SH-2X probe DNA, as characterized by Exo III digestion with 200 nM cDNA (black) or DNase I hydrolysis with (pink) or without 200 nM cDNA (blue). Error bars show standard deviations from three measurements.