Table 2. Drug and/or vaccine target prioritization parameters and functional annotation of the eight essential non-host homologous putative targets.

Gene and protein codes	Official full name	Cavities with DSa > 0.80	Cavities with DSa,g > 0.60 and < 0.80	Mol. Wtb (KDa)	Functionsc	Cellular componentd	Pathwayse	Virulencef
NP_939692.1, nusB	Transcription antitermination protein NusB/ N utilization substance protein B homolog	0	2	20.382	MF: RNA binding. BP: DNA-templated transcription, termination, regulation of transcription, DNA-templated.	Cytoplasm	unknown	No
NP_939612.1, hisE	Phosphoribosyl-ATP pyrophosphatase	0	1	9.877	MF: RNA binding, phosphoribosyl-ATP diphosphatase activity BP: histidine biosynthetic process	Cytoplasm	Biosynthesis of amino acids	Yes
NP_939445.1, DIP1084	Iron ABC transporter membrane protein/ Putative iron transport membrane protein, FecCD-family	2	3	35.470	MF: Transporter activity BP: Transport	Membrane	The ATP-binding cassette (ABC) transporters form one of the largest known protein families	Yes
NP_939345.1, DIP0983	Hypothetical protein DIP0983/ Uncharacterized protein	0	4	28.193	MF: possible lysine decarboxylases (Pfam)/52% sequence identity with PDB Template 1WEK. BP: A pyridoxal-phosphate protein. Also acts on 5-hydroxy-L-lysine (IUBMB Comments)	Cytoplasm	unknown	Yes
NP_939302.1, glpX	Fructose 1,6-bisphosphatase II	3	2	35.589	MF: fructose 1,6-bisphosphate 1-phosphatase activity, metal ion binding. BP: gluconeogenesis, glycerol metabolic process		Carbohydrate Metabolism	No
NP_939123.1, smpB	SsrA-binding protein	1	2	18.784	MF: RNA binding	Cytoplasm	unknown	Yes
NP_938900.1, rpsH	30S ribosomal protein S8	1	1	14.292	MF: rRNA binding, structural constituent of ribosome BP: Translation	Extracellula/ Cytoplasm	unknown	No
NP_938502.1, bioB	Biotin synthase	3	1	38.224	MF: 2 iron, 2 sulfur cluster binding, 4 iron 4 sulfur cluster binding, biotin synthase activity, iron ion binding BP: biotin biosynthetic process	Cytoplasm	Biotin metabolism	Yes

^aDruggability predicted with DoGSiteScorer software. A druggability score above 0.60 is considered to be good, but a score above 0.80 is favored [32].

^bMolecular weight was determined using ProtParam tool (http://web.expasy.org/protparam/).

^cMolecular function (MF) and biological process (BP) for each target protein was determined using UniProt.

^dCellular localization of pathogen targets was performed using CELLO.

^eKEGG was used to find the role of these targets in different cellular pathways.

^fPAIDB was used to check if the putative targets are involved in the pathogen’s virulence.

^gDS = Drug Score