Figure 4.

Dose–response pattern and persistence of miR214 knockdown in vivo.

Notes: *P<0.05. (A) Dose-dependent anti-miR214 knockdown determined by real-time PCR and normalized to baseline after tail-vein injection of PU–anti-miR214 or Asp⁸-PU–anti-miR214 at doses of 2–32 mg/kg; (B) persistence of miR214 knockdown examined by real-time PCR and normalized to baseline after tail-vein injection of PU–anti-miR214 or Asp⁸-PU–anti-miR214 at dose of 16 mg/kg (data presented as means ± SE, n=6 per group); (C) representative bright-field microscopy of sections from distal femora with TRAP staining in mice treated with PU-Cy³-miR214 or Asp⁸-PU-Cy³-miRNA (bars 100 μm); (D) representative bright-field microscopy of sections from distal femora with TRAP staining in mice treated with Asp⁸-PU-Cy³-miR214 (bars 100 μm).

Abbreviations: PCR, polymerase chain reaction; PU, polyurethane.