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. 2017 Oct 13;12:7469–7482. doi: 10.2147/IJN.S139775

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© 2017 Cai et al. This work is published and licensed by Dove Medical Press Limited

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Cell-specific knockdown efficiency of miR214 in vivo.

Notes: *P<0.05. (A) Real-time qPCR analysis for miR214-knockdown efficiency in OSCAR⁺ and OSCAR⁻ cells sorted from bone marrow by FACS from Ovx mice administered free anti-miR214 injection of PU–anti-miR214 and Asp⁸-PU–anti-miR214 at 24 and 48 hours after administration; (B) real-time qPCR analysis for mRNA expression of TRAP and CTSK in OSCAR⁺ and OSCAR⁻ cells sorted from bone marrow by FACS from Ovx mice administered PU–anti-miR214 and Asp⁸-PU–anti-miR214 at 48 hours after administration; (C) real-time qPCR analysis for miR214-knockdown efficiency in OSCAR⁺ and OSCAR⁻ cells sorted from bone marrow by FACS from Ovx mice pretreated with PBS or Asp⁸ followed by administration of Asp⁸-PU–anti-miR214 at 24 and 48 hours; (D) real-time qPCR analysis for mRNA expression of TRAP and CTSK in OSCAR⁺ and OSCAR⁻ cells sorted from bone marrow by FACS from Ovx mice pretreated with PBS or Asp⁸ followed by administration of Asp⁸-PU–anti-miR214 at 48 hours. All data presented as means ± SE, n=6 per group.

Abbreviations: qPCR, quantitative polymerase chain reaction; PU, polyurethane; FACS, fluorescence-activated cell sorting; OSCAR, osteoclast associated receptor; Ovx, ovariectomized; NS, not significant.

Cell-specific knockdown efficiency of miR214 in vivo.

Notes: *P<0.05. (A) Real-time qPCR analysis for miR214-knockdown efficiency in OSCAR⁺ and OSCAR⁻ cells sorted from bone marrow by FACS from Ovx mice administered free anti-miR214 injection of PU–anti-miR214 and Asp⁸-PU–anti-miR214 at 24 and 48 hours after administration; (B) real-time qPCR analysis for mRNA expression of TRAP and CTSK in OSCAR⁺ and OSCAR⁻ cells sorted from bone marrow by FACS from Ovx mice administered PU–anti-miR214 and Asp⁸-PU–anti-miR214 at 48 hours after administration; (C) real-time qPCR analysis for miR214-knockdown efficiency in OSCAR⁺ and OSCAR⁻ cells sorted from bone marrow by FACS from Ovx mice pretreated with PBS or Asp⁸ followed by administration of Asp⁸-PU–anti-miR214 at 24 and 48 hours; (D) real-time qPCR analysis for mRNA expression of TRAP and CTSK in OSCAR⁺ and OSCAR⁻ cells sorted from bone marrow by FACS from Ovx mice pretreated with PBS or Asp⁸ followed by administration of Asp⁸-PU–anti-miR214 at 48 hours. All data presented as means ± SE, n=6 per group.

Abbreviations: qPCR, quantitative polymerase chain reaction; PU, polyurethane; FACS, fluorescence-activated cell sorting; OSCAR, osteoclast associated receptor; Ovx, ovariectomized; NS, not significant.