Table 1. Peptide sequences used in this study and their physicochemical properties*.
| Sequence | Mass1 | Isoelectric point (pI)1 | Net Charge at pH 71 | Hydropathy index (h)2 | Diffusion coefficient (cm2 s−1) 3 | Size4 (L/W, nm) | |
|---|---|---|---|---|---|---|---|
| P-1 | YLGEEYVK | 999.49 | 4.26 | -1 | -5.9 | 4.37 × 10−6 | 2.3/1.0 |
| P-2 | DRVYIHPFHL | 1295.68 | 7.91 | +0.2 | -2.0 | 3.89 × 10−6 | 3.0/1.0 |
| P-3 | EAIYAAPFAKKK | 1335.76 | 10.05 | +2 | -3.6 | 3.80 × 10−6 | 3.8/1.0 |
*1
Calculated using the peptide property calculator in http://www.innovagen.com/custom-peptide-synthesis/peptide-property-calculator/peptide-property-calculator.asp;
2
Calculated by adding the hydropathy value for each amino acid residue together (Kyte, J.; Doolittle, R. F. J. Mol. Biol. 1982, 157, 105-132);
3
Calculated based on equation log D = -0.434 log MW – 4.059 (Hosoya, O.; Chono, S.; Saso, Y.; Juni, K.; Morimoto, K.; Seki, T. J. Pharm. Pharmacol. 2004, 56, 1501-1508);
4
Measured from the structure model calculated by molecular mechanics with Merck molecular force field (MMFF) in Spartan'14 (L = length; W = width)