Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 24;2(2):160–180. doi: 10.1016/j.jacbts.2016.12.002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

PP2Ce Expression Promotes Myocytes Death and Perturbs Prosurvival Signaling Following Oxidative Injury

(A) NRVM viability measured by MTT assay in NRVM expressing GFP or PP2Ce following H₂O₂ treatment for 16 h at different doses as indicated. Replicate number is labeled in each corresponding column. (B) NRVM viability expressing GFP (n = 8) or PP2Ce (n = 8) under untreated or following 24 h of treatment of 2 μmol/l ionomycin. (C) Immunoblot analysis of intracellular signaling molecules as indicated in NRVM-expressing GFP or PP2Ce with 50 μmol/l H₂O₂ treatment at different time points as indicated. Tunicamycin (5 μg/ml for 4 h) was used as a control. (D) Quantification of signaling molecules as indicated with independent experimental replicate number labeled in each column. **p < 0.01 versus untreated. ##p < 0.01 between GFP and PP2Ce groups at same doses. Abbreviations as in Figures 2, 3, and 4.

PP2Ce Expression Promotes Myocytes Death and Perturbs Prosurvival Signaling Following Oxidative Injury

(A) NRVM viability measured by MTT assay in NRVM expressing GFP or PP2Ce following H₂O₂ treatment for 16 h at different doses as indicated. Replicate number is labeled in each corresponding column. (B) NRVM viability expressing GFP (n = 8) or PP2Ce (n = 8) under untreated or following 24 h of treatment of 2 μmol/l ionomycin. (C) Immunoblot analysis of intracellular signaling molecules as indicated in NRVM-expressing GFP or PP2Ce with 50 μmol/l H₂O₂ treatment at different time points as indicated. Tunicamycin (5 μg/ml for 4 h) was used as a control. (D) Quantification of signaling molecules as indicated with independent experimental replicate number labeled in each column. **p < 0.01 versus untreated. ##p < 0.01 between GFP and PP2Ce groups at same doses. Abbreviations as in Figures 2, 3, and 4.