Characteristics Features of Compound 1 on TLC, Compound 1 observed under UV 365 nm on TLC plate. Compound 1 displayed the retention factors (Rf) of 0.27 and 0.90 on TLC plate, when petroleum ether- ethyl acetate (3:2 v/v, solvent 2) and ethyl acetate- methanol- water (35:5:4 v/v, solvent 1) were used as eluents, respectively. Compound 1 showed positive results to alkaloids (spray reagent 1), terpenoids (spray reagent 2) and phenol (spray reagent 3) detecting spray reagents on TLC plate.