Table 3.
Primers Using in VDR Genotyping and Size of PCR Product
| Gene Location | Primers | Amplicon size (bp) | |
|---|---|---|---|
| Exon 2 | FokI F (46559145-46559162) | 5’-TGGCCGCCATTGCCTCCG-3’ | |
| FokI f (46559145-46559162) | 5’-TGGCCGCCATTGCCTCCA-3 | 77 | |
| FokI C (46559204-46559221) | 5’-AGCTGGCCCTGGCACTGA-3’ | ||
| Intron8 | BsmI B(46526083-46526102) | 5’-AGCCTGAGTACTGGGAATGT-3’ | 534 |
| BsmI b(46526083-46526102) | 5’-AGCCTGAGTACTGGGAATGC-3’ | ||
| BsmI C(46526599-46526616) | 5’-GGGAGGGAGTTAGGCACC-3’ | ||
| Intron8 | ApaI A(46525104-46525123) | 5’-TGGGATTGAGCAGTGAGGT-3’ | 229 |
| ApaI a(46525104-46525123) | 5’-TGGGATTGAGCAGTGAGGG-3’ | ||
| ApaI C(46524894-46524912) | 5’-CCTCATTGAGGCTGCGCAG-3’ | ||
| Exon 9 | TaqI T(46525024-46525041) | 5’-CAGGACGCCGCGCTGATT-3’ | 148 |
| TaqI t(46525024-46525041) | 5’-CAGGACGCCGCGCTGATC-3’ | ||
| TaqI C(46524894-46524912) | 5’-CCTCATTGAGGCTGCGCAG-3’ |