Figure 7. Keratinocyte deletion of arginase-1 affects cardiovascular acclimation to hypoxia.

Mice were individually housed for 14 hr in normoxia before being exposed to hypoxia (10%) at the light-dark interphase for 48 hr. Mice then recovered for a further 24 hr in normoxia. (A) Skin temperature acclimation in littermate (black line, n = 7) and K14cre-arginase-1 (green line n = 6). Data shown as mean degree’s Celsius. Lower line graph shows temperature deviation of K14cre-arg-1 from littermate. (B) Acute and (C) chronic analysis of systolic and diastolic blood pressure in littermate (black-line and grey-line n = 6) and K14cre-arg-1 (dark green line and brown line n = 6) respectively. Data shown as mean (mm/Hg). Lower line graph shows blood pressure deviation for K14cre-arg1 compared to littermate for both chronic systolic (green line) and diastolic (brown line) BP. (D) Acute and (E) chronic analysis of heart rate in littermate (black line n = 6) and K14cre-arg-1 (green line n = 6). Data shown as mean beats per minute. Lower line graph shows chronic heart rate deviation of K14cre-arg1 from littermate. (F) Analysis of metabolic activity during the chronic acclimation of littermate (black line, n = 6) and K14cre-arg-1 (green line n = 6) to hypoxia. Data shown as mean oxygen consumption VO₂ and (G) carbon dioxide generated VCO₂ ml/Kg/hr. (H) Analysis of physical activity in littermate (black line n = 6) and K14cre-arg-1 (green line n = 6) during acclimation to chronic hypoxia. Data shown as mean activity meters/hour.