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. Author manuscript; available in PMC: 2017 Oct 19.
Published in final edited form as: Fertil Steril. 2015 Apr 22;103(6):1469–76.e1-3. doi: 10.1016/j.fertnstert.2015.02.035

Figure 4.

Figure 4

MiR-21 expression in KGN cells in response to estradiol treatment. KGN cells were treated with 10−7 and 10−8 M estradiol, respectively. Raw Ct values were normalized to RNU43 housekeeping snoRNA and relative expression was determined using the 2− Δ ΔCt method. Fold change compared to controls treated with media alone was determined. One-way ANOVA was used to determine statistical differences between groups. Expression of both miR-21-5p (A) and miR-21-3p (B) increased significantly in response to treatment with Estradiol 10−7 M. The results represent mean±SD, *p<0.05 and **p<0.001.