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. Author manuscript; available in PMC: 2017 Dec 19.
Published in final edited form as: Oncogene. 2017 Jun 19;36(42):5840–5851. doi: 10.1038/onc.2017.171

Figure 4. Lateral motility of mesenchymal-type Ncad+ cells creates an invasion-permissive cell:cell network.

Figure 4

Individual DOV13 (Ncad+) cells were applied atop 3D collagen gels (1.5mg/ml) inside a glass-bottom dish, and series of z-stack confocal microscopy images were acquired using fluorescence and reflectance modes to visualize cells (green) and collagen (blue), respectively, during the course of incubation. A) Representative images demonstrate cellular network formation via tip-like cell:cell junctions (top view) and matrix invasion (3D view) by cancer cells after 7 days of incubation. Scale bar: 100μm. B) A magnified 3D volume view depicts cellular junctions between invading cells and adjacent superficially located cells (indicated by arrows). Scale bar: 50 μm.