Table 1.

Cell lysis buffers and DNA extraction procedures of six protocols: ethanol precipitation using sodium chloride (EtSC), ethanol precipitation using ammonium acetate (EtAA), Chelex 5% (Ch5%), LaboPass^™ Genomic DNA Purification Kit (LP), DNeasy^® Blood and Tissue Kit (DN), and PowerSoil DNA Isolation Kit (PS). For each protocol, 40 samples from 10 cicada exuviae were employed

Protocol	Volume of cell lysis buffer	Volume of Proteinase K (20 mg/ml)	Incubation temperature (oC)
Ethanol precipitation using sodium chloride	600 μl of TNES buffer (Tris pH 7.5 10 mmol/L, NaCl 400 mmol/L, EDTA 100 mmol/L, and SDS 0.6%)	70 μl	50
Ethanol precipitation using ammonium acetate	600 μl of digestion buffer (NaCl 50 mmol/L, Tris pH 8.0 50 mmol/L, EDTA pH 8.0 20 mmol/L, and SDS 1%)	6 μl	55
Chelex 5%	360 μl of chelex 5% buffer	80 μl	57
LaboPass™ Genomic DNA Purification Kit	800 μl buffer TL	40 μl	56
DNeasy® Blood and Tissue Kit	360 μl buffer ATL	40 μl	56
PowerSoil DNA Isolation Kit	60 μl Solution C1	60 μl	65