Table 2.

Multiple Sidak pairwise comparisons of DNA concentration, A260/280 and A260/230 ratios across five protocols. Samples extracted by Chelex 5% were excluded due to lack of baseline buffer. The protocol was determined as a significant factor in the variation in DNA concentration, A260/280 and A260/230 ratios using generalized linear models. For each protocol, 10 exuviae, each divided into four exoskeleton parts, were randomly chosen. Significant p values are shown in bold. I–J: difference between protocol I and protocol J, SE: standard error

Protocol I	Protocol J	ln(DNA concentration)			A260/280 ratio			ln(A260/230 ratio)
		I–J	SE	p	I–J	SE	p	I–J	SE	p
Sodium chloride	Ammonium acetate	12.13	3.72	.011	0.25	0.04	<.001	0.02	0.06	1
	Labopass	17.61	3.44	<.001	−0.02	0.04	1	−0.10	0.07	.828
	DNeasy	4.71	4.36	.963	0.16	0.05	.005	−0.89	0.16	<.001
	PowerSoil	13.30	3.64	.003	−0.30	0.04	<.001	−0.76	0.13	<.001
Ammonium acetate	Labopass	5.48	2.1	.087	−0.27	0.04	<.001	−0.12	0.07	.623
	DNeasy	−7.42	3.46	.277	−0.09	0.04	.379	−0.91	0.15	<.001
	PowerSoil	1.17	2.41	1	−0.55	0.04	<.001	−0.77	0.13	<.001
Labopass	DNeasy	−12.90	3.09	<.001	0.18	0.04	.001	−0.79	0.16	<.001
	PowerSoil	−4.31	1.95	.243	−0.28	0.04	<.001	−0.65	0.14	<.001
DNeasy	PowerSoil	8.59	3.31	.091	−0.46	0.04	<.001	0.14	0.19	.999