Table 1.
Success rate (%) in different strata of the data—that is, the number of samples producing sequence data (after processing by the bioinformatic pipeline) divided by the total number of samples in each group. COI and 16S refer to data retrieved by each primer pair. We found no significant difference between the number of successful samples for COI and 16S or different DNA extraction methods
| Nr of samples | COI % | 16S % | |
|---|---|---|---|
| Extraction method | |||
| NucleoSpin Tissue XS Kit | 24 | 66.7 | 95.8 |
| Salt extraction method | 24 | 75.0 | 100 |
| Zymo Research Fecal DNA Micro Kit | 24 | 50.0 | 91.7 |
| Predator species | |||
| Enallagma cyathigerum | 24 | 79.2 | 100 |
| Lestes sponsa | 24 | 62.5 | 95.8 |
| Sympetrum danae | 24 | 50.0 | 91.7 |
| Sex | |||
| Females | 36 | 61.1 | 94.4 |
| Males | 36 | 66.7 | 97.2 |