TABLE 3.
Targeted evaluation of Verigene BC-GN and MALDI Sepsityper for 170 monomicrobial cultures
| Rapid ID test | Definitive ID and resistance markera | No. of cultures | Sensitivity |
Specificity |
||
|---|---|---|---|---|---|---|
| TP/(TP + FN)b | PPA (95% CI) | TN/(TN + FP)b | NPA (95% CI) | |||
| Verigene BC-GN | Escherichia coli | 82 | 82/82 | 100 (96.5–100) | 88/88 | 100 (95.8–100) |
| CTX-M | 6 | 6/6 | 100 (61–100) | 164/164 | 100 (97.7–100) | |
| Klebsiella pneumoniaec | 31 | 30/31 | 96.8 (83.8–99.4) | 139/140 | 99.3 (96.1–99.9) | |
| CTX-M | 2 | 2/2 | 100 (34.2–100) | 168/168 | 100 (97.8–100) | |
| Pseudomonas aeruginosa | 21 | 21/21 | 100 (84.5–100) | 149/149 | 100 (97.5–100) | |
| Proteus spp. | 10 | 10/10 | 100 (72.3–100) | 160/160 | 100 (97.7–100) | |
| Enterobacter spp. | 17 | 17/17 | 100 (81.6–100) | 151/153 | 98.7 (95.4–99.6) | |
| Acinetobacter spp. | 5 | 5/5 | 100 (56.6–100) | 165/165 | 100 (97.7–100) | |
| Klebsiella oxytoca | 3 | 3/3 | 100 (43.9–100) | 167/167 | 100 (97.8–100) | |
| Citrobacter spp. | 1 | 1/1 | 100 (20.7–100) | 169/169 | 100 (97.8–100) | |
| Total | 170 | 169/170 | 99.4 (96.7–99.9) | 1,188/1,191 | 99.8 (99.3–99.9) | |
| MALDI Sepsityper | Escherichia colid | 82 | 80/82 | 97.6 (91.5–99.3) | 88/88 | 100 (95.8–100) |
| Klebsiella pneumoniaee | 31 | 30/31 | 96.8 (83.8–99.4) | 138/140 | 98.6 (94.9–99.6) | |
| Pseudomonas aeruginosa | 21 | 21/21 | 100 (84.5–100) | 149/149 | 100 (97.5–100) | |
| Proteus spp.f | 10 | 9/10 | 90 (59.6–98.2) | 160/160 | 100 (97.7–100) | |
| Enterobacter spp.g | 17 | 15/17 | 88.2 (65.7–96.7) | 153/153 | 100 (97.6–100) | |
| Acinetobacter spp. | 5 | 5/5 | 100 (56.6–100) | 165/165 | 100 (97.7–100) | |
| Klebsiella oxytoca | 3 | 3/3 | 100 (43.9–100) | 157/157 | 100 (97.6–100) | |
| Citrobacter spp.h | 1 | 1/1 | 100 (20.7–100) | 167/169 | 98.8 (95.8–99.7) | |
| Total | 170 | 164/170 | 96.5 (92.5–98.4) | 1,177/1,191 | 98.8 (98.0–99.3) | |
a
With the exception of CTX-M, no other drug resistance markers were detected.
b
TP, true positive; TN, true negative; FP, false positive; FN, false negative.
c
False positive sample was BC157; false negative sample was BC172.
d
False negative samples were BC61 and BC142.
e
False negative sample was BC192; false positive samples were BC04 and BC160.
f
False negative sample was BC41.
g
False negative samples were BC68 and BC102.
h
False positive samples were BC68 and BC102.