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. 2017 Aug 23;55(9):2785–2800. doi: 10.1128/JCM.00634-17

FIG 1.

FIG 1

HIV-1 genetic diversity of the study subjects. (A) Pie chart showing the HIV-1 subtype distribution of our study population according to the phylogenetic analysis of the rt sequences and according to HIV BLAST (https://www.hiv.lanl.gov); (B) phylogenetic tree of rt sequences (HIV region from positions 2723 to 3225 according to HXB2 numbering) of the study population generated with Sanger sequencing (red) and next-generation sequencing (NGS) (green), including longitudinal time points, together with reference sequences (black) from the Los Alamos sequence database (https://www.hiv.lanl.gov). For the NGS analysis, consensus sequences were generated for each longitudinal time point per study subject using DNASTAR's SeqMan Pro. Neighbor-joining phylogenetic trees were generated using MEGA and FigTree software. The number following the study subject's identifier represents the sample collection time point. The black bar indicates the genetic distance. Subject MDC192 (gray asterisk) is either CRF02_AG or CRF36_cpx.