TABLE 1.
Selected drug resistance mutations for comparative ARMS-PCR, Sanger sequencing, and NGS analyses
| ARV class | Mutation(s)a | Prevalence (%) | Drug(s) affected | Mutation score |
|---|---|---|---|---|
| NRTI | K65R | TDF | 60 (high-level resistance) | |
| M184V | 39–77.2 | 3TC, FTC | 60 (high-level resistance) | |
| T215Y/F | 11–24 | AZT | 45 (low-level resistance) | |
| NNRTI | K103N | 14–44 | NVP, EFV | 60 (high-level resistance) |
| Y181C | 18–19.8 | NVP | 60 (high-level resistance) |
a
The selection of drug resistance mutations for ARMS-PCR, Sanger sequencing, and NGS was based on (i) the prevalence of the mutations in Cameroon (22, 47, 51, 52), (ii) the drugs deployed in Cameroon and affected by the mutation, and (iii) the mutation score (http://hivdb.stanford.edu/DR/asi/releaseNotes/index.html).