TABLE 2.
Primers used in ARMS-PCR to detect the K65R, M184V, T215Y/F, K103N, and Y181C mutationsa
| ARV class | Mutation(s) | Nature of mutation or primer name | Primer sequence | HXB2 location | Product length (bp) |
|---|---|---|---|---|---|
| NRTI | K65R | WT | 5′-CTAATTTTCTCCATTTAGTACTATCTTTAT-3′ | 2772–2743 | 185c |
| Mut | 5′-CTAATTTTCTCCATTTAGTACTATCTTGTC-3′ | 2772–2743 | |||
| M184V | WT | 5′-TAAATCAGATCCTACATACAAATCATACAT-3′ | 3128–3099 | 328b | |
| Mut | 5′-TAAGTCAGATCCTACATATAAATCATCCTC-3′ | 3128–3099 | |||
| T215Y/F | WT | 5′-TTCTTTCTGATGTTTCTTATCTGGTGTGGT-3′ | 3221–3192 | 421b | |
| Mut | 5′-TTCTTTCTGATGTTTCTTATCTGGTGGGWA-3′ | 3221–3192 | |||
| Common forward 1 | 5′-CTCAAGACTTCTGGGAGGTCT-3′ | 2800–2820 | |||
| NNRTI | K103N | WT | 5′-TCTCCCACATCCAGTACTGTTACTGATGTT-3′ | 2887–2858 | 300c |
| Mut | 5′-TCTCCCACATCCAGTACTGTTACTGAGTTR-3′ | 2887–2858 | |||
| Y181C | WT | 5′-ATCCTACATATAAATCATCCACATATTGRT-3′ | 3120–3091 | 533c | |
| Mut | 5′-ATCCTACATATAAATCATCCACATATGGRC-3′ | 3120–3091 | |||
| Common forward 2 | 5′-AGCCAGGAATGGATGGCCCAA-3′ | 2587–2607 |
a
WT, wild-type reverse primer; Mut, mutant reverse primer; HXB2 location, primer location according to the HIV-1 subtype B reference sequence.
b
The product length when combined with that of Common forward1.
c
The product length when combined with that of Common forward2.