Table 1.
Comparison of ViroCap filter elution using a peristaltic pump and manual bilge pump (elution device) method
| Variable | Peristaltic pump | Elution device |
|---|---|---|
| Biosafety and cross-contamination potential | ||
| Receiving vessel | Open 125-mL bottle | 100-mL specimen container |
| Non-autoclavable parts in BSC | Peristaltic pump | None |
| Use and specifications | ||
| Setup time (min:s) | 1:15 | 1:41 |
| Active elution time (min) | 23 | 26 |
| Power mechanism | Electric | Manual |
| Weight (kg) | 8.0 | 4.1 |
| Surface area inside BSC (cm2) | 840 | 510 |
| Surface area outside BSC (cm2) | 0 | 260 |
| Height (cm) | 16 | 29 |
| Cost | ||
| Materials and manufacturing cost for 10 samplesa | ~ US$1800 | ~ US$505–$555/$650–$750b,c |
| Efficiency and recovery | ||
| PV1 yield, ± AD (%) | 17.1 ± 4.6 (n = 6) | 17.3 ± 2.3 (n = 6) |
| Max pump pressure drop (mmHg) | N/A | 72 (1.4 psi) |
| Average elution volume, ± AD (mL) | 100 ± 6.0 (n = 6) | 97 ± 5.2 (n = 6) |
BSC biosafety cabinet, PV1 poliovirus type 1, N/A not applicable, AD average deviation, n number, psi pounds per square inch
aAssumes that reusable supplies (e.g., tubing, collection cups) are not disinfected between samples, and 10 complete sets of supplies are included in the cost
bProduction at scale/single unit
cRange provides minimum and maximum estimated values