Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 19;7:13575. doi: 10.1038/s41598-017-14148-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Stimulation of recombinant TFPI-2 (rTFPI-2) inhibits proliferation and invasion of breast cancer cells. (A) ELISA assays indicate the relative levels of secreted TFPI-2 in the culture medium of stable MDA231 cell lines. Data were analyzed from three independent experiments.*P < 0.05 as determined by Student’s t-test. (B) ELISA assays show that the levels of TFPI-2 in culture medium of MDA231 cells were higher than that in MCF7 cells (~6.85 fold). Data were analyzed from three independent experiments. **P < 0.01 as determined by Student’s t-test. (C) Effect of exogenously offered rTFPI-2 on proliferation of MCF7 cells. MCF7 cells were cultured in the medium containing various concentrations of rTFPI-2 (0, 6.25, 12.5, 25, 50ng/ml). Cell viability was measured by MTT assays at the time of 2, 24, 48, and 72hrs. P < 0.05. (D) Transwell assays show the invasive capacity of MCF7 cells after incubation with exogenous rTFPI-2 (0, 12.5, 25, 50ng/ml). Representative images of the assays were shown and statistical data were analyzed from three independent experiments. *P < 0.05 as determined by one-way ANOVA followed by Tukey’s multiple comparison tests.