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. 2017 Oct 19;7:13612. doi: 10.1038/s41598-017-13497-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SH-SY5Y cells as a model for the heterogeneity of NB. (a) Phase contrast images displaying the heterogeneity of NB cells within and across different cell lines as indicated (scale bar = 100 µm; indicates an N-type, an S-type NB cell present in the SH-SY5Y cell line, right panel). (b) Flow cytometric screen of the expression levels of 242 surface molecules on SH-SY5Y cells. The heat map graph illustrates the percentage of positive cells for specific antigens within the overall live cell population (color scale; n = 1). Boxes shaded in white represent a ≤ 5% cut-off threshold value. CD: cluster of differentiation. (c) Expression of surface marker candidates selected for subsequent analysis under serum-free conditions (n ≥ 3; error bars represent standard deviation). (d) Immunofluorescence analysis confirming surface marker expression patterns and cellular heterogeneity of the SH-SY5Y NB line (scale bar = 20 µm).

Inline graphic — SH-SY5Y cells as a model for the heterogeneity of NB. (a) Phase contrast images displaying the heterogeneity of NB cells within and across different cell lines as indicated (scale bar = 100 µm; indicates an N-type, an S-type NB cell present in the SH-SY5Y cell line, right panel). (b) Flow cytometric screen of the expression levels of 242 surface molecules on SH-SY5Y cells. The heat map graph illustrates the percentage of positive cells for specific antigens within the overall live cell population (color scale; n = 1). Boxes shaded in white represent a ≤ 5% cut-off threshold value. CD: cluster of differentiation. (c) Expression of surface marker candidates selected for subsequent analysis under serum-free conditions (n ≥ 3; error bars represent standard deviation). (d) Immunofluorescence analysis confirming surface marker expression patterns and cellular heterogeneity of the SH-SY5Y NB line (scale bar = 20 µm).