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. 2017 Oct 19;7:13612. doi: 10.1038/s41598-017-13497-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detecting distinctive responses of NB subpopulations to specific small molecule modulators. (a) Flow cytometry analysis of SPADE-defined NB subpopulation responsiveness to small molecule modulators following 48 hours of treatment (concentrations as indicated in Supplementary Table S2). In the small molecule screen conducted, four reagents revealed the capacity for enhancing or decreasing a specific subpopulation. Specifically, BMP4 was able to enhance neuronal differentiation whilst decreasing the amount of CD15^high/CD24^high/CD57^high/CD184^high cells (Cluster 1). Error bars represent standard deviation. Dashed lines represent 1 standard deviation from DMSO control. *p ≤ 0.05 in a one-way ANOVA test followed by Dunnett’s multiple comparisons test comparing all columns to the DMSO control. (b) List of small molecules applied in the screen. Colored arrowheads highlight up-, or downregulation of subpopulations induced by the respective molecules (n ≥ 3).

Inline graphic — Detecting distinctive responses of NB subpopulations to specific small molecule modulators. (a) Flow cytometry analysis of SPADE-defined NB subpopulation responsiveness to small molecule modulators following 48 hours of treatment (concentrations as indicated in Supplementary Table S2). In the small molecule screen conducted, four reagents revealed the capacity for enhancing or decreasing a specific subpopulation. Specifically, BMP4 was able to enhance neuronal differentiation whilst decreasing the amount of CD15^high/CD24^high/CD57^high/CD184^high cells (Cluster 1). Error bars represent standard deviation. Dashed lines represent 1 standard deviation from DMSO control. *p ≤ 0.05 in a one-way ANOVA test followed by Dunnett’s multiple comparisons test comparing all columns to the DMSO control. (b) List of small molecules applied in the screen. Colored arrowheads highlight up-, or downregulation of subpopulations induced by the respective molecules (n ≥ 3).