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. 2017 Oct 19;7:13533. doi: 10.1038/s41598-017-13524-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Uptake of CR-KP NSSTI isolates by murine macrophage-like cells. KP strains were incubated with the murine macrophage-like cell line J774.A1 at an MOI of 10. At 1 h post-infection, amikacin was added to the medium, and cells were incubated for 1 h to eradicate extracellular bacteria. The number of intracellular bacteria was then measured by lysing the eukaryotic cells and plating for viable CFUs. The results are expressed as a percentage of the inoculum, and the means and standard deviations are indicated. p values were derived from comparisons of each group to the MGH78578 group via one-way ANOVA with Bonferroni’s multiple comparison correction (*P < 0.05). Each symbol represents the mean of an assay performed in triplicate. Results were combined from three independent experiments.