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. 2017 Oct 19;7:13533. doi: 10.1038/s41598-017-13524-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Virulence of CR-KP NSSTI isolates in a subcutaneous infection model. C57BL/6 mice were infected subcutaneously with approximately 5 × 10⁶ CFU of the indicated KP strains. (A) Abscess areas were measured up to 96 h post-infection. Data are expressed as means ± SEM. p values were derived from comparisons of each group to the MGH78578 infected group at specified time points (one-way ANOVA with Bonferroni’s multiple comparison correction; *p ≤ 0.05). Bacterial burdens in the abscess (B) and the livers (C) were determined at 96 h post-infection (hpi). Each symbol represents the bacterial CFU recovered from a single mouse. Solid bars denote the median CFU, and the dashed line indicates the limit of detection (100 CFU). p values were derived from the comparisons of each group to the MGH78578 infected group via the Mann-Whitney U test. *p ≤ 0.05. Data are combined from 3 independent experiments (n = 9 for each group).