Figure 5.

Persistence and dissemination of CR-KP NSSTI isolates following neutrophil depletion in the subcutaneous infection model. C57BL/6 mice treated with either anti-ly6G antibody or IgG isotype control antibody were infected subcutaneously with approximately 5 × 10⁶ CFU of the indicated KP strains. (A) Abscess areas were measured at 96 h post-infection. Data are expressed as means ± SEM. Bacterial burdens in the abscesses (B) and livers (C) were measured at 96 h post-infection. Each symbol represents the bacterial numbers recovered from a single mouse. Solid bars denote the median CFU, and the dashed line indicates the limit of detection (100 CFU). p values were derived from comparisons of each group of mice to the MGH78578 infected groups via one-way ANOVA with Bonferroni’s multiple comparison correction (abscess area) or the Mann-Whitney U test (CFU); *p ≤ 0.05 vs. IgG control group; ⁺p ≤ 0.05 vs. Ly6G treated group. For comparisons within each strain, p values were derived via Student’s t test (^#p ≤ 0.05). Data are combined from 3 independent experiments (n = 9 for each group).