Fig. 1.

Reduction in SLN expression prolongs the lifespan of mdx:utr ^−/− mice. a The body weight of mdx:utr ^−/− :sln ^+/− and tKO mice in comparison to that of age- and sex- matched WT and mdx:utr ^−/− control mice. We used 3–4 month old mice for this experiment. The n number for each group is shown within the bar. Data are presented as mean ± SEM. **significantly different from other groups (p < 0.005, t-test with Welch’s correction). b Kaplan–Meier survival curves indicate that the mdx:utr ^−/− :sln ^+/− and tKO mice has increased lifespan in comparison to that of mdx:utr ^−/− controls as determined by the nonparametric log-rank test; p < 0.0001. c Increased Ca²⁺ dependent SR Ca²⁺ uptake, d increased V _max of Ca²⁺ uptake and e decreased EC₅₀ values in the diaphragm of mdx:utr ^−/− :sln ^+/− and tKO mice indicates improved SERCA function. The n number for each group is shown within the bar. Data are presented as mean ± SEM. **significantly different from other groups (p < 0.0005, t-test with Welch’s correction). *significantly different from WT and mdx:utr ^−/− mice (p < 0.005, t-test with Welch’s correction). f–h Representative western blot analysis and quantitation of SLN, SERCA1, SERCA2a and CSQ proteins in the diaphragm, pectoral and quadriceps muscles respectively. Tissues are from 3 to 4 month old mice. Uncropped scans of western blots are shown in Supplementary Fig. 8a–c. Data are represented as mean ± SEM. The n number for each group is shown within the bar diagram. *significantly different from other groups, p < 0.05, t-test with Welch’s correction