Fig. 2.

Reduction in SLN expression ameliorates muscle pathology. a Calpain activity is restored to normal levels in the pectoral muscles of mdx:utr ^−/− :sln ^+/− and tKO mice. Data are presented as mean ± SEM (t-test with Welch’s correction) of five independent experiments performed in duplicates. The n number for each group is shown within the bar. b Representative H&E and Masson’s trichrome stained quadriceps and diaphragm muscles. Arrow indicates increased mononuclear infiltration (indicative of necrosis) and collagen (blue) accumulation (indicative of fibrosis) in mdx:utr ^−/− mice. Original magnification is ×20. Scale bar=100 μm. c–f Quantitation show that the necrotic and fibrotic areas were significantly reduced in both diaphragm and quadriceps of mdx:utr ^−/− :sln ^+/− mice and in the quadriceps of tKO mice in comparison to that of mdx:utr ^−/− controls. The n number for each group and the p values (t-test with Welch’s correction) are shown within the graph. Data are presented as mean ± SEM. Tissues from 3 to 4 month old mice are used for all the above experiments