Fig. 4.

STARs allow for control of multi-gene metabolic pathways. a Schematic of the four gene deoxyviolacein metabolic pathway (vioABCE) that converts L-tryptophan into the purple compound deoxyviolacein. b Schematic DNA template of a plasmid containing a STAR regulated vioABCE pathway. Characterization was performed on E. coli DH5 alpha pir cells transformed with this plasmid in the absence (-STAR) and presence (+STAR) of a plasmid encoding cognate STAR. Deoxyviolacein production was characterized by solvent extraction and spectral quantification of deoxyviolacein (measured in units of OD at 575 nm). Data represent mean values and error bars represent s.d. of n = 9 biological replicates