Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 19;7:13609. doi: 10.1038/s41598-017-13599-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Serotonergic neurons in the dorsal raphe nucleus (DRN) show spontaneous firing activity. (a) Representative cropped image of single-cell reverse transcription polymerase chain reaction after whole-cell recording. Tryptophan hydroxylase 2 (Tph2) mRNA was used as a marker of serotonergic neurons. Glutamate decarboxylase 1 and 2 mRNA (Gad1 and Gad2), markers of GABAergic neurons, were used as negative controls. Gamma-enolase mRNA (Eno2), a marker for neurons, was used as a positive control. Uncropped image was shown in Supplementary Fig. S1. (b) Representative trace of the action potential (AP) recorded from DRN serotonergic neurons. Serotonergic neurons showed a wide action potential and a long-lasting after hyperpolarization. (c) Representative phase plane plot of membrane potential vs. its derivative with respect to time (dV/dt). Five APs from one neuron was plotted. (d) Representative membrane voltage histogram. The higher voltage peak was considered as pseudo resting membrane potential (RMP). (e) Electrophysiological characters of 22 serotonergic neurons from 7 mice. Recordings were performed in normal ACSF condition without any drug or electrical stimulation. AHP; afterhyperpolarization. (f) Time course of recording the effects of drug perfusion. Spontaneous firing was recorded for 30 s before and after drug application, and changes in the firing rate were calculated. (g,h) Representative traces of the spontaneous firing before (left) and after (right) the application of DNQX (20 μM), APV (50 μM) and bicuculline (20 μM) (g) or prazosin (1 μM) (h). (i) The changes in the spontaneous firing rate before and after the application of DNQX (20 μM), APV (50 μM), and bicuculline (20 μM), or prazosin (1 μM). (DNQX + APV + bicuculline, n = 4 neurons from 3 mice, P = 0.2545 by paired t-test; prazosin, n = 3 neurons from 2 mice, P = 0.0855 by paired t-test). Data are presented as the mean ± S.E.M.