FIG 8.

Overexpression of bacterioferritin partly restores colony growth in Fur-depleted cells. Shown is the colony (A) and planktonic (B) growth of PAO1 and conditional fur mutant cells carrying the empty vector pME6032 or the bacterioferritin-expressing vector pMEbfrB in MH broth (or on MH agar) supplemented with 0.5 mM IPTG at 37°C. Images in panel A were taken after 24 or 40 h of growth and are representative of three experiments with similar results. Values in panel B are the mean (±standard deviation) from two independent experiments performed in duplicate. ara, arabinose.