TABLE 1.
Kinetic parameters of Fe-S d-iLDH and FAD-containing dehydrogenase domain toward different substratesa
| Substrate | Fe-S d-iLDH |
FAD-containing dehydrogenase domain |
||||
|---|---|---|---|---|---|---|
| kcat (s−1) | Km (μM) | kcat/Km (μM−1 · s−1) | kcat (s−1) | Km (μM) | kcat/Km (μM−1 · s−1) | |
| d-Lactate | 120 ± 19 | 57 ± 7.8 | 2.1 | 17 ± 1.5 | 94 ± 11 | 0.176 |
| l-Lactate | 9.5 ± 1.1 | 1,040 ± 50 | 9 × 10−3 | 1.5 ± 0.3 | 1,780 ± 270 | 0.84 × 10−3 |
| d-2-Hydroxybutyrate | 66 ± 5.0 | 100 ± 5.3 | 0.66 | 13 ± 0.65 | 170 ± 16 | 0.078 |
| d-Glycerate | 8.0 ± 1.4 | 1,380 ± 140 | 6 × 10−3 | 0.78 ± 0.1 | 1,670 ± 140 | 0.47 × 10−3 |
a
All experiments were carried out with 0.2 mM MTT as the electron acceptor. Values are the mean ± SD from three parallel replicates.