TABLE 3.
Kinetics of Fe-S d-iLDH and FAD-containing dehydrogenase domain with different electron acceptorsa
| Electron acceptor | Fe-S d-iLDH |
FAD-containing dehydrogenase domain |
||||
|---|---|---|---|---|---|---|
| kcat (s−1) | Km (μM) | kcat/Km (μM−1 · s−1) | kcat (s−1) | Km (μM) | kcat/Km (μM−1 · s−1) | |
| MTT | 128 ± 5.7 | 48 ± 1.9 | 2.7 | 5.3 ± 0.97 | 31 ± 4.1 | 0.17 |
| Coenzyme Q10 | 2.4 ± 0.029 | 4.1 ± 0.35 | 0.59 | ND | ND | ND |
| Cytochrome c | 0.49 ± 0.04 | 51.5 ± 1.7 | 9.5 × 10−3 | 2.0 ± 0.07 | 9.5 ± 0.33 | 0.21 |
a
All experiments were carried out with 1.0 mM d-lactate as the substrate. ND, not detected. kcat and Km values are the mean ± SD from three parallel replicates.