TABLE 4.
Iron-to-protein ratio of purified Fe-S oxidoreductase domain and its derivates
| Proteina | Iron-to-protein ratiob |
|
|---|---|---|
| Before reconstitution | After reconstitution | |
| Fe-S oxidoreductase domain | 2.84 ± 0.035 | 17.71 ± 0.62 |
| M-1 | 2.80 ± 0.12 | 14.03 ± 0.31 |
| M-2 | 1.73 ± 0.12 | 13.58 ± 0.61 |
| M-4 | 0.79 ± 0.047 | 10.16 ± 0.17 |
| M-6 | 0.26 ± 0.017 | 8.07 ± 0.38 |
| M-7 | 0.033 ± 0.0024 | 4.51 ± 0.32 |
a
M-1, M-2, M-4, M-6, and M-7 stand for C540S, C540S-C594S, C540S-C594S-C751S-C752S, C540S-C594S-C751S-C752S-C540S-C594S, and C540S-C543S-C594S-C751S-C752S-C540S-C594S mutated Fe-S oxidoreductase domains, respectively.
b
Values are the mean ± SD from three parallel replicates.