FIG 2.

Growth of asparaginase mutants on different carbon and nitrogen sources. The bacterial strains indicated were grown on M9 minimal medium lacking ammonium chloride and supplemented with the following carbon and nitrogen sources: 5 mM glucose only (no nitrogen source) (A), 5 mM glucose with 5 mM ammonium chloride (B), 5 mM glucose plus 5 mM l-asparagine (C), and 5 mM F-Asn only (D). All data points are the means from triplicate cultures measured on two different occasions (6 total points). Error bars represent standard deviations.