TABLE 1.

Strains and plasmids used

Strain or plasmid	Genotype or descriptiona	Reference, source, or construction
Strains
14028	Wild-type Salmonella enterica subspecies enterica serovar Typhimurium	ATCC
HMB106	14028 ΔansA80::Kan	Lambda Red mutation of ansA made using PCR primers BA3004 and BA3005
HMB107	14028 ΔansB80::Kan	Lambda Red mutation of ansB made using PCR primers BA3006 and BA3007
HMB174	14028 ΔansA80::Kan	P22 transduction of ΔansA80::Kan from HMB106 into a clean 14028 background
HMB175	14028 ΔansB80::Kan	P22 transduction of ΔansB80::Kan from HMB107 into a clean 14028 background
HMB188	14028 ΔfraBDA80	6
HMB195	14028 ΔfraE4	6
HMB196	14028 ΔfraD4	6
HMB203	14028 ΔfraE4 ΔansA80::Kan	P22 transduction of ΔansA80::Kan from HMB106 into HMB195
HMB204	14028 ΔfraE4 ΔansB80::Kan	P22 transduction of ΔansB80::Kan from HMB107 into HMB195
HMB206	14028 ΔfraB80::Kan	6
HMB207	14028 ΔfraE4 ΔansA80	Antibiotic cassette in HMB203 was flipped out using pCP20
HMB208	14028 ΔfraE4 ΔansB80	Antibiotic cassette in HMB204 was flipped out using pCP20
HMB210	14028 ΔfraE4 ΔansA80 ΔansB80::Kan	P22 transduction of ΔansB80::Kan from HMB107 into HMB207
HMB212	14028 ΔansA80	Antibiotic cassette in HMB174 was flipped out using pCP20
HMB213	14028 ΔansB80	Antibiotic cassette in HMB175 was flipped out using pCP20
HMB214	14028 ΔfraE4 ΔansA80 ΔansB80	Antibiotic cassette in HMB210 was flipped out using pCP20
HMB215	14028 Δfra80	6
HMB218	14028 ΔfraBD81	6
HMB220	14028 ΔansA80 ΔansB80::Kan	P22 transduction of ΔansB80::Kan from HMB107 into HMB212
HMB248	14028 ΔfraA4	6
Plasmids
pKD46	PBAD gam bet exo pSC101 oriTS (Ampr)	16
pKD4	FRT-kan-FRT oriR6K (Ampr)	16
pCP20	cI857 λPR flp pSC101 oriTS (Ampr Camr)	20

^aAmp^r, ampicillin resistance; Cam^r, chloramphenicol resistance.