FIG 2.

Identification of 5′ and 3′ termini of pheU and pheV transcripts by cDNA cloning and sequencing of circularized RNAs in various genetic backgrounds, as described in Materials and Methods. The mature sequences are identical for pheU and pheV and are represented as a rectangle. The 5′ upstream sequences are also identical for pheU and pheV, and the two transcription initiation sites, as determined in this study (Fig. 1C and D), are underlined. The 3′ sequences downstream of CCA are distinct for pheU and pheV and are shown in parallel. Each arrow (down or up) represents a terminus, as determined from the cDNA sequencing. Numbers above or below the arrow are numbers of 3′ or 5′ ends identified by sequencing. An asterisk indicates that some 3′ ends at a particular location contained untemplated poly(A) tails of 1 to 3 nt.