FIG 5.

Analysis of the processing of the valV valW polycistronic transcript. (A) Schematic presentation of the valV valW transcript. Previously identified RNase P-processing sites (P) (3) are shown above the cartoon. The position of the oligonucleotide probe (c) is shown below the cartoon. The diagram is not drawn to scale. (B) Northern blot of the valV valW transcript probed with the oligonucleotide probe (c) that hybridizes to mature sequences of both valV and valW (3). Northern analysis was conducted as described in Materials and Methods. The processing intermediates identified previously (3) are shown to the right of the image. The genotypes of the strains used are indicated above each lane. Unrelated lanes were removed from the image (dotted lines).