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. 2017 Oct 19;7:13544. doi: 10.1038/s41598-017-12881-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Prediction of ligand-binding sites on the CD300b extracellular domain. Schematic representation of the five predicted binding sites (3DLigandSite) within the extracellular domain of human CD300b. The amino acids defining each cluster are listed and shown in blue within the structure. Heterogens (glycan chains and ions) used to build the models are also shown. The amino acids highlighted in red were subjected to mutagenesis to assess their role in complex establishment.