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. 2017 Oct 19;7:13544. doi: 10.1038/s41598-017-12881-8

Figure 7.

Figure 7

Expression of CD300f on MS patient’s monocytes. (A) Gating strategy to define the three populations of peripheral monocytes. Single cell monocytic population was gated by size and complexity. Next, we selected the HLA-DR positive population. Then, three populations were defined based on the expression of CD14 and CD16; Classical Monocytes (CD14hi/CD16neg), Intermediate Monocytes (CD14hi/CD16pos) and Non-Classical Monocytes (CD14lo/CD16pos). We analyzed the level of expression of CD300f and CD300e on the three monocytic populations. (B) Analysis of the levels expression of CD300f and CD300e on monocytes from healthy individuals and MS patients. Graphs show the mean fluorescent intensity of CD300f and CD300e expressed on the cell surface of peripheral monocytes from 11 controls and 21 MS patients. Statistically significant differences between groups were determined by a two-tail Mann-Whitney U test: * ≤ 0.05, ** ≤ 0.01.