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. 2017 Oct 19;7:13532. doi: 10.1038/s41598-017-13206-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of pharmacological intervention on renal cortical Oat3 function and expression. [³H]ES uptake calculated from tissue/medium ratio. (A) Western blot analysis of Oat3 expression in whole cell lysate fraction normalized by β-actin (B) and in membrane lysate fraction (cropped blots) normalized by β-actin (C). Na⁺-K⁺ ATPase was used as a marker for the membrane fraction. Full-length blots are presented in Supplementary Figure 1. Bar graphs presented show mean ± SEM. n = 6 rats per group. C - control group; DM - diabetic group; DMI - diabetic plus insulin group; DMIS - diabetic and insulin plus atorvastatin group; DMS - diabetic plus atorvastatin group; CS - control plus atorvastatin. *p < 0.05 vs. control group and control plus atorvastatin groups, ^†p < 0.05 vs. diabetic group, ^‡p < 0.05 vs. diabetic plus insulin group, and ^#p < 0.05 vs. diabetic plus atorvastatin group.