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. 2017 Oct 17;83(21):e01182-17. doi: 10.1128/AEM.01182-17

FIG 1.

FIG 1

Polymicrobial biofilm tests of P. aeruginosa and E. faecalis. (A) Colony observation of cocultured P. aeruginosa and E. faecalis (red arrows). The growth experiment of P. aeruginosa (gray triangle) and E. faecalis (black square), grown individually (B) or together (C), in a shaking incubator at 37°C. The viable count assay was used to determine the CFU per milliliter. (D) The CV biofilm assay of the monospecies and the dual-species biofilms. *, P < 0.001 versus the biofilm levels of PAO1 and PAO1 plus E. faecalis (PAEF) or E. faecalis (EF). (E) Viscosity measurements of the mono- and dual-species biofilms. Viscosity appears in units of centipoise (cP). *, P < 0.0001 versus viscosity of PAO1 and PAEF or EF. (F) SEM images of the mono- and dual-species biofilms of P. aeruginosa and/or E. faecalis. The magnifications are ×5,000. (G) Images of the mono- and dual-species biofilms of P. aeruginosa and/or E. faecalis dripping out of the 96-well plates. Detailed video clips are provided in the movies in the supplemental material.