TABLE 2.

Primers used in this study

Mutation/gene target by primer type	Flanking region	Direction	Primer sequence (5′–3′)a	Restriction enzyme
Primers for clean deletion
alg	Left	Forward	ACCTTGAGCTCGCATGGGTCGAAGATTAAGG	SacI
	Left	Reverse	CGTTAATGAGGTGGCCGTATAAGTCGAAGTAGAGCTGCGC
	Right	Forward	GCGCAGCTCTACTTCGACTTATACGGCCACCTCATTAACG
	Right	Reverse	TAGAGGAGCTCTCTGCAATGGCTGGTTGTAG	SacI
psl	Left	Forward	ACCTTGCATGCGGGCTGGTACATCCAGAAGA	SphI
	Left	Reverse	TCGTCGATAGTGGCTTTGTGAGCATTCCGACAAGGAGC
	Right	Forward	GCTCCTTGTCGGAATGCTCACAAAGCCACTATCGACGA
	Right	Reverse	TAGAGGCATGCGCATCGACCTGAAAATCCTC	SphI
pelA	Left	Forward	ACCTTGAGCTCCGATCATCCTCGGCTTTCT	SacI
	Left	Reverse	CAAAACCTGTCGCGTAGTGGTAATCGCTCATCCACAGC
	Right	Forward	GCTGTGGATGAGCGATTACCACTACGCGACAGGTTTTG
	Right	Reverse	TAGAGGAGCTCCGCTGGGCATGAATACTTCT	SacI
qRT-PCR primers
pelA		Forward	GGT GAT TAT GTT CCA GGC ACT
		Reverse	GGT GAA CCA GAA GAT CAC CA
pslB		Forward	TGG CTG ACC TTC AAC AGC GA
		Reverse	TGC TCG AAG TCA CCG AGC TT
16S rRNA		Forward	CTT ACG GCC AGG GCT ACA CA
		Reverse	GTA CAA GGC CCG GGA ACG TA

^aRestriction enzyme recognition sites are underlined.