TABLE 2.

Plasmids and primers used in this study

Primer name	Sequence (5′ to 3′)a	Useb
MG-for	ACAAGATCTTATGGACAGTTGCGGA	Cloning of Erm
MG-rev	TATGGATCCAGCTACCAAGACGAAG	Cloning of Erm
PDH-phomo-for	ATCTCTAGAGGCTGACCCATACAAGCA	Cloning of pdhk
PDH-phomo-rev	CATAAGCTTCCACAATATCCATCCCATC	Cloning of pdhk
POX-phomo-for	ATTTCTAGAACCGTGGAAGGACTTTATTTG	Cloning of poxk
POX-phomo-rev	TTCAAGCTTCGTTTCACCAGTCGCAATC	Cloning of poxk
M13 Primer RV	CAGGAAACAGCTATGAC	Verification of gene inactivation
PDH-RT-for	CAAATGATTCAACACGGGACT	Quantitative PCR
PDH-RT-rev	TAAGCAAAGGCGACACGAT	Quantitative PCR
POX-RT-for	GCCTGGTGCGACCCATCTA	Quantitative PCR
POX-RT-rev	TGGCGTTTCATCCATTTCCTG	Quantitative PCR
16S-RT-for	CGGCGTATTAGTTAGTTGGTG	Quantitative PCR
16S-RT-rev	TTCCCTACTGCTGCCTCCC	Quantitative PCR

^aRestriction sites in the primer sequences are underlined.

^bErm, erythromycin resistance gene; pdhk, homogeneous fragment of pdh; poxk, homogeneous fragment of pox.