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SatA acetylates streptothricin once. (A) Reaction mixtures of [1-14C]acetyl-CoA, streptothricin, and H6-BsSatA were incubated at 37°C for 1 h and then spotted onto a thin-layer chromatography (TLC) silica plate. The plate was developed for 1 to 2 h, and label distribution was visualized using a phosphor imager. (B) Quantification of the amount of acetylated streptothricin as a function of the amount of AcCoA consumed using a continuous spectrophotometric assay.
