BsSatA is a dimer in solution. (A) H6-BsSatA retention data for gel permeation analysis. The retention time of H6-BsSatA was compared to times of a mixture of standards used to generate a calibration curve. Standards used were γ-globulin (158 kDa), ovalbumin (44 kDa), myoglobin (17 kDa), and vitamin B12 (1.35 kDa), which were purchased from Bio-Rad Laboratories. (B) Calculated molecular masses and oligomers for BsSatA and BsSatA with streptothricin, indicating that BsSatA was likely a dimer in solution.