FIG 7.

Binding of substrates to BsSatA. ITC was used to analyze the binding of streptothricin and AcCoA to BsSatA. (A and B) Binding isotherms for streptothricin or AcCoA to BsSatA. The top panels are the raw data from consecutive injections of ligand represented as heat release over injection time. The bottom panels are binding isotherms obtained by integration of the raw data. Data were acquired with a TA Instruments NanoITC, and the data were analyzed with NanoAnalyze 1.2 software. The streptothricin titration (A) displayed saturation, while AcCoA titration (B) showed no binding of AcCoA to BsSatA.