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Effect of autoclaving time on H2O2 concentration in autoclaved agar. Bacto agar (15 g liter−1) was autoclaved in 0.5, 3, or 20 mM phosphate buffer (Na2HPO4-KH2PO4 [pH 7.0 ± 0.15]) at 121°C for 15 or 90 min. Data are means from five independent experiments ± SD.
