TABLE 3.
Primers used in this study
| Primer | Sequence (5′ to 3′) | Target | PCR(s)a |
|---|---|---|---|
| LMOSA_2150F | GATCTGCACTGCGTTCTTC | LMOSA_2150 | 1, 8 |
| LMOSA_2150R | GAAGCCCAGCAAACTTATCG | LMOSA_2150 | 1, 12, 21, 22 |
| LMOSA_2180F | CTGGATCAAAATTGCGGAC | LMOSA_2180 | 2 |
| LMOSA_2180R | TACCACTAATTGAACAAGCC | LMOSA_2180 | 2, 8 |
| LMOSA_2220F | CAACTTTGACCCTGTGGAG | LMOSA_2220 | 3, 9 |
| LMOSA_2220R | CTTTCCATTCAATCACTGCG | LMOSA_2220 | 3, 13 |
| pLI37_F | CAACCAGATCAGTTACCATTAAC | LMOSA_2260 | 4 |
| pLI37_R | TGCTTCTCCAGAGATTTCTTCTG | LMOSA_2260 | 4, 9 |
| LMOSA_2330F | GCATACGTACGAACCAGAAG | LMOSA_2330 | 5 |
| LMOSA_2330R | CAGTGTTTCTGCTTTTGCTCC | LMOSA_2330 | 5 |
| LMOSA_2400F | GTCGTTTGCTTGATAGAGGCG | LMOSA_2400 | 6, 10 |
| LMOSA_2400R | GGGCGATGGTTTGAACTACC | LMOSA_2400 | 6 |
| LMOSA_2450F | TAATAGCCAGCAACTCCAGC | LMOSA_2450 | 7, 14, 22 |
| LMOSA_2450R | GAAAAAGACCGCTTGTCTCTG | LMOSA_2450 | 7, 10 |
| F2365_2257F | ACATTGCGAGAACACCTTGG | LMOf2365_2257 | 11, 12, 13 |
| F2365_2257R | GATTTATCGGCGCAATGACG | LMOf2365_2257 | 11, 14 |
| LMOSLCC2372_2751F | TAACCAATAAGCCAACACCG | LMOSLCC2372_2751 | 15 |
| LMOSLCC2372_2751R | CTTCTTTCCACTTCCCGAGC | LMOSLCC2372_2751 | 15 |
| OLM10_1F | ATCCGCCATTGCACAGAGAG | LMOSA_2150 homolog in OLM 10 | 16, 19 |
| OLM10_1R | CGTGCGGTGGCTCAAATTTT | LMOSA_2150 homolog in OLM 10 | 16, 20 |
| OLM10_2F | GCGGAGAAATTGGTGAACGG | Cystathionine γ-synthase gene in OLM 10 | 17 |
| OLM10_2R | ACAACTAGCCCTGCGGTTAC | Cystathionine γ-synthase gene in OLM 10 | 17, 19 |
| OLM10_3F | GAGTAGGGAACGTAAGCCCG | LMOSA_2450 homolog in OLM 10 | 18 |
| OLM10_3R | CCGCTGGCTCCTTTCAGTTA | LMOSA_2450 homolog in OLM 10 | 18 |
| Marq207 | GGCCACGCGTCGACTAGTACNNNNNNNNNNGTAAT | Random primer with adaptor (first amplification) | 21 |
| Marq208 | GGCCACGCGTCGACTAGTAC | Primer annealing to the adaptor region of Marq207 (second, nested amplification) | 21 |
| LMOSA_2150R2 | GCTATGTTACAGGAATGGCG | LMOSA_2150 | 21 |
| LMOSA_2470F | GGAAGTCAGAGATAAGCTG | LMOSA_2470 | 21,22 |
| LMOSA_2470F2 | CTTAGAAGAACTGGACTCTG | LMOSA_2470 | 21 |
| F2365_0072F | GACTAAGTTGCCGAGTGAAC | LMOf2365_0072 | 22 |
| F2365_0072R | ATCGTGTCAGCAACATGTGG | LMOf2365_0072 | 22 |
| F2365_0271F | CGTTACCCATGGTGATGCTG | LMOf2365_0271 | 22 |
| F2365_0271R | CGCTGATTTGGAATGTTTTAG | LMOf2365_0271 | 22 |
| F2365_0902F | CCGTTTTCCGCGCCATTAATAG | LMOf2365_0902 | 20, 22 |
| F2365_0902R | GCAGTAGTAACTTTGACCGC | LMOf2365_0902 | 22 |
| F2365_2335F | CACTAGTCGGAATATCCCTC | LMOf2365_2335 | 22 |
| F2365_2335R | GCGGAATATATGGAGACTTC | LMOf2365_2335 | 22 |
| F2365_2381F | CTCGCGCTCTCTAAGTAATTC | LMOf2365_2381 | 22 |
| F2365_2382R | CTGGTAAAGGCCAACGTCAAG | LMOf2365_2382 | 22 |
| F2365_2418F | CTGCTTGGTGCGATAAAATC | LMOf2365_2418 | 22 |
| F2365_2418R | GATACAGTTCCCTTACCAGC | LMOf2365_2418 | 22 |
| F2365_2679F | CTGAACAGCAGACTCGTAAG | LMOf2365_2679 | 22 |
| F2365_2679R | GAACGGATGCAGATGTTTGGC | LMOf2365_2679 | 22 |