FIG 2.

Validation of predicted biomass yields. (A) Validation versus experimental biomass yields in shake-flask cultures using media with various ratios of glutamate, lactate, and ammonia, as obtained from Thalen et al. (10). (B) Validation of predicted versus experimental biomass yields in pH-controlled batch fermentations using newly formulated media (Data Set S5). All simulations were made with a partially dysfunctional TCA cycle.