TABLE 3.

Fermentation medium composition^a

Compound	Concn (mg/liter)
	MSS-CDM	CIT-NH3	IMP-CDM	IMP2-CDM	IMP2-CDM-AA as in MSS-CDM
l-Proline	1,040	0	882	882	1,040
Na-l-glutamate	20,000	0	18,677	18,677	18,677
l-Cysteine HCl	40	0	4	0	0
Na2S2O3·5H2O	0	5.65	0	2.83	2.83
NaCl	2,500	0	73	73	73
KH2PO4	500	500	500	500	500
KCl	200	200	200	200	200
MgCl2·6H2O	100	1,000	1,000	1,000	1,000
CaCl2·2H2O	20	20	20	20	20
FeSO4·7H2O	10	0	0	0	0
Fe(III)-citrate·3H2O	0	20	20	20	20
Tris	6,100	0	0	0	0
CuCl2·2H2O	0	1.28	1.28	1.28	1.28
CoCl2·6H2O	0	0.42	0.42	0.42	0.42
ZnCl2	0	10	10	10	10
MOPS	0	2,500	2,500	2,500	2,500
Ascorbic acid	400	623	623	623	623
Reduced glutathione	150	233	233	233	233
Niacin	4	6	6	6	6
Dimethyl-β-cyclodextrin	1,000	1,000	1,000	1,000	1,000
Na acetate	0	0	409	409	409
l-Alanine	312	0	304	304	312
l-Aspartic acid	436	0	524	524	436
l-Glutamic acid	1,600	0	3,475	3,475	3,475
l-Histidine	188	0	32	32	188
l-Glycine	163	0	149	149	163
l-Isoleucine	288	0	244	244	288
l-Leucine	484	0	438	438	484
l-Lysine HCl	600	0	393	393	600
l-Methionine	156	0	116	116	156
l-Phenylalanine	250	0	234	234	250
l-Serine	230	0	187	187	230
l-Tyrosine	67	0	34	34	67
l-Valine	456	0	399	399	456
Thiamine-HCl	0	10	10	10	10
Biotin	0	0.2	0.2	0.2	0.2
Riboflavin	0	0.3	0.3	0.3	0.3
Calcium pantothenate	0	4	4	4	4
Citric acid monohydrate	0	26,268	0	0	0
Ammonium hydroxide 25%	0	5,066	0	0	0

^apH adjustment was made to pH 7.4 (25°C) with 5 M NaOH and 6 M HCl.